{"id":94,"date":"2023-09-25T00:29:17","date_gmt":"2023-09-24T17:29:17","guid":{"rendered":"https:\/\/seacoast.kmutt.ac.th\/seateams2026\/?page_id=94"},"modified":"2026-08-16T13:57:21","modified_gmt":"2026-08-16T06:57:21","slug":"past-schools","status":"publish","type":"page","link":"https:\/\/seacoast.kmutt.ac.th\/seateams2026\/past-schools\/","title":{"rendered":"Past Schools"},"content":{"rendered":"

Conceptual Course in Molecular Structure Determination 2025<\/b><\/h4><\/div>
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\u201cI\u2019ve learned a great deal of fundamentals in understanding structural biology techniques. I now appreciate the importance of crystal structures and the theory behind crystallization. I\u2019ve gained hands-on knowledge in refining models using Cryo-EM and X-ray crystallography, and I\u2019ve come to understand concepts like twinning, electron density interpretation, and how to improve atomic models. I also learned how to differentiate atoms not supported by electron density and gained practical experience in protein and ligand fitting \u2014 all of which are essential for accurate structure determination and analysis\u201d<\/i><\/p>\n<\/div><\/div><\/blockquote>

Feedback from the 2025 participants<\/strong><\/span><\/div><\/div>
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\u201cBefore studying macromolecular crystallography, I had a general basic idea that it involved using X-rays to determine the structure of biological molecules. However, I learned many deeper and more specific aspects that I hadn\u2019t known before. For example, I didn\u2019t realize how crucial crystal growth is to the entire process. Proteins must be crystallized in just the right conditions\u2014pH, temperature, salt concentration.\u201d<\/i><\/p>\n<\/div><\/div><\/blockquote>

Feedback from the 2025 participants<\/strong><\/span><\/div><\/div>
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\u201cInitially, I had no knowledge about cryo-electron microscopy (Cryo-EM). However, through this learning experience, I\u2019ve developed a foundational understanding of its principles and workflows. I\u2019ve learned how Cryo-EM differs from X-ray crystallography, especially in sample preparation, data collection, and structure determination. I\u2019m now able to interpret Cryo-EM maps, assess resolution, and perform model fitting using tools like Coot and Moorhen, particularly for ligand fitting. This progress has given me the confidence to distinguish between Cryo-EM and X-ray crystallography in both theory and practical application. I also recognize that there is still much more to learn, and I look forward to deepening my knowledge on these techniques.\u201d<\/i><\/p>\n<\/div><\/div><\/blockquote>

Feedback from the 2025 participants<\/strong><\/span><\/div><\/div>
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\u201cBefore studying cryo-electron microscopy, I had a basic understanding used to visualize biological molecules at high resolution. However, through the course, I discovered several key aspects that were completely new to me and deepened my appreciation for the technique.\u201d<\/i><\/p>\n<\/div><\/div><\/blockquote>

Feedback from the 2025 participants<\/strong><\/span><\/div><\/div>
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\u201cIn X-ray crystallography, I\u2019ve begun to understand the basic concepts such as symmetry, rotational, and the overall importance of phasing in structure determination. While I still find some aspects especially phasing and symmetry a bit challenging,. I believe more guided examples or case studies would be helpful and also examples involving protein-ligand complexs\u00a0 would be helpful to strengthen my understanding and improve practical application in this area.\u201d<\/i><\/p>\n<\/div><\/div><\/blockquote>

Feedback from the 2025 participants<\/strong><\/span><\/div><\/div>
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\u201cThe course was informative and offered a solid foundation in macromolecular crystallography Including a few case studies where crystallography played a key role in solving a biological or medical question would help students understand the real-world applications and impact of the technique.\u201d<\/i><\/p>\n<\/div><\/div><\/blockquote>

Feedback from the 2025 participants<\/strong><\/span><\/div><\/div>
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\u201c<\/i>The course has been truly satisfied and enriched the expectations. The team members were patient, supportive, and dedicated in guiding me throughout the sessions, and I sincerely appreciate their efforts. I would also like to thank Dr. Leela and Dr. Ruslan for organizing and leading this course\u00a0 their commitment made this learning experience possible. One suggestion for improvement would be to enhance the tutorials by providing clearer explanations and placing more emphasis on the use of indicators during model building,molecular replacements and structure refinement. I also understand that each structure presents unique challenges, so including a wider range of case examples would be helpful to reflect the diversity in structure-solving approaches.\u201d<\/i><\/p>\n<\/div><\/div><\/blockquote>

Feedback from the 2025 participants<\/strong><\/span><\/div><\/div><\/div>
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